Hotspot

atpF-atpH

LSC · 14449-15001 · 553 bp

Back to Calophyllum

Pi 0.0109

MarkerSeek score 68.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 553
variable_sites: 9
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	CGGAGGGAAAAATACGAGGG	50.0	55.0	58.0	58.1	696-707 bp	1.0	82.3
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	CGGAGGGAAAAATACGAGGG	50.0	55.0	58.3	58.1	723-734 bp	1.0	80.4
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	TGAGGCGGAGGGAAAAATAC	50.0	50.0	58.0	57.6	701-712 bp	1.0	79.3
atpF-atpH_p4	TTAAATCCGAAACTCCCGGC	TGAGGCGGAGGGAAAAATAC	50.0	50.0	58.3	57.6	728-739 bp	1.0	77.5
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	697-708 bp	1.0	77.0