Hotspot

trnS-GCU-trnG-UCC

LSC · 8672-10528 · 1857 bp

Back to Calophyllum

Pi 0.00767

MarkerSeek score 52.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1857
variable_sites: 20
pi: 0.00767
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AGTCCACTCAGCCATCTCTC	CATCGTTAGCTTGGAAGGCT	55.0	50.0	58.5	58.0	1944-1983 bp	1.0	78.7
trnS-GCU-trnG-UCC_p2	AGTCCACTCAGCCATCTCTC	CGTTAGCTTGGAAGGCTAGG	55.0	55.0	58.5	58.1	1941-1980 bp	1.0	78.5
trnS-GCU-trnG-UCC_p3	AGTCCACTCAGCCATCTCTC	TTGCTTTCATTCGGCTCCTT	55.0	45.0	58.5	58.1	1859-1898 bp	1.0	78.4
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	TTTGCTTTCATTCGGCTCCT	55.0	45.0	58.5	58.1	1860-1899 bp	1.0	78.4
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	GTTAGCTTGGAAGGCTAGGG	55.0	55.0	58.5	57.7	1940-1979 bp	1.0	76.3