Hotspot

trnH-GUG-psbA

LSC · 190-599 · 410 bp

Back to Calophyllum

Pi 0.00825

MarkerSeek score 47.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 5
pi: 0.00825
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	469-479 bp	1.0	81.1
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	852-862 bp	1.0	80.1
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.1	58.0	851-861 bp	1.0	80.1
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	TGCATGGTTCCTTGGTAACC	55.0	50.0	58.1	58.1	843-853 bp	1.0	79.8
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TAGCTGCTTGGCCTGTAGTA	55.0	50.0	58.1	58.1	660-670 bp	1.0	79.8