Hotspot

clpP

Genome · 70773-72073 · 1301 bp

Back to Calophaca

Pi 0.0216

MarkerSeek score 74.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 1301
variable_sites: 27
pi: 0.0216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GCCCTCGGCTCACATTTTT	GTCTCCTTACGTTCTCACACC	52.6	52.4	58.7	58.1	1347-1385 bp	1.0	62.2
clpP_p2	GCCCTCGGCTCACATTTTT	AGACAACCGCATCTTCATCT	52.6	45.0	58.7	56.9	1316-1354 bp	1.0	62.2
clpP_p3	GCCCTCGGCTCACATTTTT	CTCCTTACGTTCTCACACCA	52.6	50.0	58.7	56.9	1345-1383 bp	1.0	61.8
clpP_p4	GCCCTCGGCTCACATTTT	AGACAACCGCATCTTCATCT	55.6	45.0	58.0	56.9	1316-1354 bp	1.0	60.1
clpP_p5	GCCCTCGGCTCACATTTT	GTCTCCTTACGTTCTCACACC	55.6	52.4	58.0	58.1	1347-1385 bp	1.0	60.0