Hotspot

trnN-GUU-trnV-GAC

Genome · 100175-100579 · 405 bp

Back to Calophaca

Pi 0.0128

MarkerSeek score 65.00

Primer available yes

Label trnN-GUU-trnV-GAC

Feature Metadata

label_name: trnN-GUU-trnV-GAC
feature_type: igs
region: Genome
length_bp: 405
variable_sites: 5
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnV-GAC_p1	TGGTCGTAGGTTCGAATCCT	CTCCATTCGATCCCGTCATC	50.0	55.0	58.2	57.9	539-553 bp	1.0	81.0
trnN-GUU-trnV-GAC_p2	TGGTCGTAGGTTCGAATCCT	GATGACTTCCACCACGTCAA	50.0	50.0	58.2	57.8	472-486 bp	1.0	80.6
trnN-GUU-trnV-GAC_p3	TGGTCGTAGGTTCGAATCCT	ATGACTTCCACCACGTCAAG	50.0	50.0	58.2	57.8	471-485 bp	1.0	80.6
trnN-GUU-trnV-GAC_p4	TGGTCGTAGGTTCGAATCCT	AACTGATGACTTCCACCACG	50.0	50.0	58.2	57.8	476-490 bp	1.0	80.5
trnN-GUU-trnV-GAC_p5	CGATTGGTCGTAGGTTCGAA	CTCCATTCGATCCCGTCATC	50.0	55.0	57.7	57.9	543-557 bp	1.0	80.1