Hotspot

rps19-rpl2

Genome · 85042-85656 · 615 bp

Back to Calophaca

Pi 0.16

MarkerSeek score 98.40

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: Genome
length_bp: 615
variable_sites: 93
pi: 0.16
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 98.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGTTCTGGACCAAG	TTGATTCTTCGTCGCCGTAG	47.6	50.0	57.0	58.1	760-1705 bp	1.0	71.6
rps19-rpl2_p2	GGTAGATGTTCTGGACCAAGT	TTGATTCTTCGTCGCCGTAG	47.6	50.0	57.0	58.1	759-1704 bp	1.0	71.6
rps19-rpl2_p3	TGGTAGATGTTCTGGACCAAG	GGGCCCCAATTGGTAGAAAA	47.6	50.0	57.0	58.1	852-1797 bp	1.0	71.4
rps19-rpl2_p4	GGTAGATGTTCTGGACCAAGT	GGGCCCCAATTGGTAGAAAA	47.6	50.0	57.0	58.1	851-1796 bp	1.0	71.4
rps19-rpl2_p5	TGGTAGATGTTCTGGACCAAG	TTGGGGTTATCCTGCACTTG	47.6	50.0	57.0	57.8	818-1763 bp	1.0	70.3