Hotspot

rps3-rps19

Genome · 84447-84762 · 316 bp

Back to Calophaca

Pi 0.019

MarkerSeek score 50.00

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: Genome
length_bp: 316
variable_sites: 6
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CCTTCTCTGAGCCATTCGAC	CGGACGAGATGGTTGTTTCT	55.0	50.0	58.1	57.9	1419 bp	0.5	58.8
rps3-rps19_p2	TATCCGTCCAATTCCCTCGA	CGGACGAGATGGTTGTTTCT	50.0	50.0	57.9	57.9	1063 bp	0.5	58.7
rps3-rps19_p3	ACGTCCCGCAATTTGTACTT	CGGACGAGATGGTTGTTTCT	45.0	50.0	58.1	57.9	1375 bp	0.5	58.5
rps3-rps19_p4	CCCTACCTTCTCTGAGCCAT	CGGACGAGATGGTTGTTTCT	55.0	50.0	58.2	57.9	1424 bp	0.5	57.8
rps3-rps19_p5	CGTCCCGCAATTTGTACTTG	CGGACGAGATGGTTGTTTCT	50.0	50.0	57.8	57.9	1374 bp	0.5	57.4