Hotspot

psbT-psbN

Genome · 74376-74435 · 60 bp

Back to Calophaca

Pi 0.0357

MarkerSeek score 74.60

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: Genome
length_bp: 60
variable_sites: 2
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AGAGAACCCCCTAAAGTTCCA	CCATTCGAAGAACATGGGGA	47.6	50.0	58.0	57.6	123 bp	1.0	72.6
psbT-psbN_p2	AGAGAACCCCCTAAAGTTCCA	TCCATTCGAAGAACATGGGG	47.6	50.0	58.0	57.6	124 bp	1.0	72.5
psbT-psbN_p3	AGAGAACCCCCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	47.6	50.0	58.0	57.4	149 bp	1.0	70.7
psbT-psbN_p4	GAACCCCCTAAAGTTCCAACT	CCATTCGAAGAACATGGGGA	47.6	50.0	57.5	57.6	120 bp	1.0	68.5
psbT-psbN_p5	GAACCCCCTAAAGTTCCAACT	TCCATTCGAAGAACATGGGG	47.6	50.0	57.5	57.6	121 bp	1.0	68.5