Hotspot

rps12-clpP

Genome · 70179-70772 · 594 bp

Back to Calophaca

Pi 0.00735

MarkerSeek score 53.10

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 594
variable_sites: 4
pi: 0.00735
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACATTTCGGATTGGCTGT	GGTAGCGGAATCTTTGTGA	45.0	47.4	57.8	54.9	668 bp	0.5	25.7
rps12-clpP_p2	TGTGACATTTCGGATTGGCT	GGTAGCGGAATCTTTGTGA	45.0	47.4	57.8	54.9	670 bp	0.5	25.7
rps12-clpP_p3	TTTCGGATTGGCTGTCTTGT	GGTAGCGGAATCTTTGTGA	45.0	47.4	57.7	54.9	663 bp	0.5	25.1
rps12-clpP_p4	TTCGGATTGGCTGTCTTGTG	GGTAGCGGAATCTTTGTGA	50.0	47.4	58.5	54.9	662 bp	0.5	23.5
rps12-clpP_p5	ACATTTCGGATTGGCTGTCT	GGTAGCGGAATCTTTGTGA	45.0	47.4	57.5	54.9	666 bp	0.5	23.3