Hotspot

petA-psbJ

Genome · 62556-63619 · 1064 bp

Back to Calophaca

Pi 0.00565

MarkerSeek score 62.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1064
variable_sites: 6
pi: 0.00565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	AACTGGTATTCCCGTGATCG	50.0	50.0	57.8	57.4	1251-1264 bp	1.0	73.8
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	AACTGGTATTCCCGTGATCG	55.0	50.0	58.3	57.4	1256-1269 bp	1.0	73.3
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	AACTGGTATTCCCGTGATCG	55.0	50.0	58.4	57.4	1260-1273 bp	1.0	72.8
petA-psbJ_p4	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGATCATCC	50.0	50.0	57.8	57.1	1201-1214 bp	1.0	71.5
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGATCATCC	55.0	50.0	58.3	57.1	1206-1219 bp	1.0	71.0