Hotspot

trnQ-UUG-accD

Genome · 56853-57457 · 605 bp

Back to Calophaca

Pi 0.0314
MarkerSeek score 71.90
Primer available yes
Label trnQ-UUG-accD

Feature Metadata

label_name
trnQ-UUG-accD
feature_type
igs
region
Genome
length_bp
605
variable_sites
19
pi
0.0314
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-accD_p1 GAGGTTCGAATCCTTCCGTC AGGAAATTCCGTATGTTCCTGA 55.0 40.9 58.1 57.2 713-715 bp 1.0 58.8
trnQ-UUG-accD_p2 GAGGTTCGAATCCTTCCGTC AGGAAATTCCGTATGTTCCTGAA 55.0 39.1 58.1 57.8 713-715 bp 1.0 56.1
trnQ-UUG-accD_p3 GAGGTTCGAATCCTTCCGTC GGAAATTCCGTATGTTCCTGA 55.0 42.9 58.1 55.7 712-714 bp 1.0 54.9
trnQ-UUG-accD_p4 GAGGTTCGAATCCTTCCGTC AGGAAATTCCGTATGTTCCTG 55.0 42.9 58.1 55.7 713-715 bp 1.0 54.8
trnQ-UUG-accD_p5 TTCGGAGGTTCGAATCCTTC AGGAAATTCCGTATGTTCCTGA 50.0 40.9 57.3 57.2 717-719 bp 1.0 54.1