Hotspot

atpF-atpA

Genome · 51698-51773 · 76 bp

Back to Calophaca

Pi 0.0395

MarkerSeek score 70.40

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: Genome
length_bp: 76
variable_sites: 3
pi: 0.0395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	TGTTTGGGGCGATGAAAGAA	ATGCCATCGCCTACTTGAAG	45.0	50.0	58.0	58.0	224 bp	1.0	80.7
atpF-atpA_p2	TTAGAGGGAGCTCTGGGAAC	ATGCCATCGCCTACTTGAAG	55.0	50.0	58.1	58.0	303 bp	1.0	79.4
atpF-atpA_p3	CTTAGAGGGAGCTCTGGGAA	ATGCCATCGCCTACTTGAAG	55.0	50.0	57.8	58.0	304 bp	1.0	79.1
atpF-atpA_p4	ATGTTTGGGGCGATGAAAGA	ATGCCATCGCCTACTTGAAG	45.0	50.0	57.8	58.0	225 bp	1.0	79.0
atpF-atpA_p5	GGTATGTTTGGGGCGATGAA	ATGCCATCGCCTACTTGAAG	50.0	50.0	58.2	58.0	228 bp	1.0	78.8