Hotspot

trnS-UGA-psbC

Genome · 26875-27098 · 224 bp

Back to Calophaca

Pi 0.0405

MarkerSeek score 64.10

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 224
variable_sites: 9
pi: 0.0405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GGGTTCGAATCCCTCTCTCT	TAGGTCATTTATGGCACGCG	55.0	50.0	58.2	58.4	352-354 bp	1.0	77.4
trnS-UGA-psbC_p2	GGGTTCGAATCCCTCTCTCT	GTAGGTCATTTATGGCACGC	55.0	50.0	58.2	57.0	353-355 bp	1.0	72.4
trnS-UGA-psbC_p3	GGGTTCGAATCCCTCTCTCT	TCGTAGGTCATTTATGGCACG	55.0	47.6	58.2	58.2	355-357 bp	1.0	71.3
trnS-UGA-psbC_p4	GGGTTCGAATCCCTCTCTCT	CGTAGGTCATTTATGGCACG	55.0	50.0	58.2	56.7	354-356 bp	1.0	70.4
trnS-UGA-psbC_p5	TTCGAATCCCTCTCTCTCCT	TAGGTCATTTATGGCACGCG	50.0	50.0	56.9	58.4	349-351 bp	1.0	70.1