Hotspot

ycf80

Genome · 52513-53931 · 1419 bp

Back to Caloglossa

Pi 0.282

MarkerSeek score 79.60

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1419
variable_sites: 565
pi: 0.282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	GTGGGTTCAATTCCCTCCAG	TCCTCCAGGAGAACATGGTC	55.0	55.0	58.2	58.4	2113 bp	0.333333	48.6
ycf80_p2	ATGGACGTGGGTTCAATTCC	TCCTCCAGGAGAACATGGTC	50.0	55.0	58.2	58.4	2119 bp	0.333333	48.6
ycf80_p3	AGGTTCAAATCCTTCCCTGC	TCCTCCAGGAGAACATGGTC	50.0	55.0	57.8	58.4	2430 bp	0.333333	48.1
ycf80_p4	GGTTCAAATCCTTCCCTGCT	TCCTCCAGGAGAACATGGTC	50.0	55.0	57.8	58.4	2429 bp	0.333333	48.1
ycf80_p5	GTGGGTTCAATTCCCTCCAG	TAGCGCCAACAATTCCTTCT	55.0	45.0	58.2	57.5	1901 bp	0.333333	48.1