Hotspot

ycf29

Genome · 151266-151886 · 621 bp

Back to Caloglossa

Pi 0.236

MarkerSeek score 80.20

Primer available yes

Label ycf29

Feature Metadata

label_name: ycf29
feature_type: gene
region: Genome
length_bp: 621
variable_sites: 201
pi: 0.236
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf29_p1	ACTGAAAACACACGATAAGGA	GGGTTCGAGTCCCGTTATTC	38.1	55.0	55.2	58.1	1331 bp	0.333333	22.3
ycf29_p2	ACTGAAAACACACGATAAGGA	GGATTGTGGCTCCATCACTC	38.1	55.0	55.2	58.3	1353 bp	0.333333	20.2
ycf29_p3	CTGAAAACACACGATAAGGATCA	GGGTTCGAGTCCCGTTATTC	39.1	55.0	56.9	58.1	1330 bp	0.333333	19.9
ycf29_p4	ACTGAAAACACACGATAAGGATC	GGGTTCGAGTCCCGTTATTC	39.1	55.0	56.9	58.1	1331 bp	0.333333	19.9
ycf29_p5	ACTGAAAACACACGATAAGGA	GGTTCGAGTCCCGTTATTCG	38.1	55.0	55.2	58.4	1330 bp	0.333333	19.3