Hotspot

ycf23

Genome · 44370-45206 · 837 bp

Back to Caloglossa

Pi 0.294

MarkerSeek score 82.60

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: Genome
length_bp: 837
variable_sites: 333
pi: 0.294
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	TGGCTGTACTCAATGCGTAC	TTCTTGTTGCAGCCCTTACT	50.0	45.0	58.0	57.3	1635 bp	0.333333	48.0
ycf23_p2	ATTGGCTGTACTCAATGCGT	TTCTTGTTGCAGCCCTTACT	45.0	45.0	57.9	57.3	1637 bp	0.333333	47.2
ycf23_p3	GATGGCTGTAAAGCTGGACA	TTCTTGTTGCAGCCCTTACT	50.0	45.0	57.9	57.3	1577 bp	0.333333	47.2
ycf23_p4	CTTGGGATGGCTGTAAAGCT	TTCTTGTTGCAGCCCTTACT	50.0	45.0	57.9	57.3	1582 bp	0.333333	47.0
ycf23_p5	TTGGGATGGCTGTAAAGCTG	TTCTTGTTGCAGCCCTTACT	50.0	45.0	58.2	57.3	1581 bp	0.333333	46.8