Hotspot

ycf21

Genome · 35383-35928 · 546 bp

Back to Caloglossa

Pi 0.269

MarkerSeek score 77.60

Primer available yes

Label ycf21

Feature Metadata

label_name: ycf21
feature_type: gene
region: Genome
length_bp: 546
variable_sites: 207
pi: 0.269
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf21_p1	TCTGCAATATAGCCGCTGTT	ACCCTTTCCTGGAGAAGACA	45.0	50.0	57.4	57.9	1440 bp	0.333333	47.4
ycf21_p2	TTCTGCAATATAGCCGCTGT	ACCCTTTCCTGGAGAAGACA	45.0	50.0	57.4	57.9	1441 bp	0.333333	47.4
ycf21_p3	TCTGCAATATAGCCGCTGTT	TGGCAGACATAGCACCTACT	45.0	50.0	57.4	58.1	1021 bp	0.333333	47.3
ycf21_p4	TTCTGCAATATAGCCGCTGT	TGGCAGACATAGCACCTACT	45.0	50.0	57.4	58.1	1022 bp	0.333333	47.3
ycf21_p5	TCTGCAATATAGCCGCTGTT	TGGGCACACTGGAAACTTAG	45.0	50.0	57.4	57.7	1275 bp	0.333333	46.2