Hotspot

syfB

Genome · 153494-155485 · 1992 bp

Back to Caloglossa

Pi 0.292

MarkerSeek score 78.00

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 1992
variable_sites: 814
pi: 0.292
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	AGGAAAGTCCGGGCTCTATT	GGGTGTAGCCAAGTGGTAAG	50.0	55.0	58.1	57.9	2833 bp	0.333333	51.5
syfB_p2	AGGAAAGTCCGGGCTCTATT	GGTGTAGCCAAGTGGTAAGG	50.0	55.0	58.1	57.9	2832 bp	0.333333	51.5
syfB_p3	AGGAAAGTCCGGGCTCTATT	GGTAAGGCAACGGACTTTGA	50.0	50.0	58.1	58.1	2819 bp	0.333333	51.5
syfB_p4	AGGAAAGTCCGGGCTCTATT	GAATCCTCCCACCCCAGATA	50.0	55.0	58.1	57.9	2780 bp	0.333333	51.4
syfB_p5	ACTATGAGGAGAGTGCCACA	GCTCATCATTCACCTGCTCA	50.0	50.0	57.8	58.0	2979 bp	0.333333	51.2