Hotspot

rnz

Genome · 16219-16899 · 681 bp

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Pi 0.281

MarkerSeek score 79.60

Primer available yes

Label rnz

Feature Metadata

label_name: rnz
feature_type: gene
region: Genome
length_bp: 681
variable_sites: 269
pi: 0.281
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rnz_p1	CAGCTGGATCATGGGCTATA	TCACGACCGTTTTTAGTGCT	50.0	45.0	56.5	57.8	1690 bp	0.333333	39.8
rnz_p2	TATAGCAGCTGGATCATGGG	TCACGACCGTTTTTAGTGCT	50.0	45.0	56.5	57.8	1695 bp	0.333333	39.8
rnz_p3	AACGTAAAGGTGGCAAACAA	TCACGACCGTTTTTAGTGCT	40.0	45.0	56.4	57.8	1889 bp	0.333333	38.6
rnz_p4	ACGTAAAGGTGGCAAACAAA	TCACGACCGTTTTTAGTGCT	40.0	45.0	56.4	57.8	1888 bp	0.333333	38.6
rnz_p5	TTATAGCAGCTGGATCATGGG	TCACGACCGTTTTTAGTGCT	47.6	45.0	57.3	57.8	1696 bp	0.333333	37.8