Hotspot

dsbD

Genome · 21165-21920 · 756 bp

Back to Caloglossa

Pi 0.31

MarkerSeek score 81.80

Primer available yes

Label dsbD

Feature Metadata

label_name: dsbD
feature_type: gene
region: Genome
length_bp: 756
variable_sites: 319
pi: 0.31
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dsbD_p1	TGGTCGTAGGTTCAAGTCCT	CGAGAAAACTTCCAGCGTCT	50.0	50.0	58.0	58.2	1244 bp	0.333333	51.6
dsbD_p2	TGGTCGTAGGTTCAAGTCCT	GCCAAACGACCAAACAATCC	50.0	50.0	58.0	58.2	1409 bp	0.333333	51.5
dsbD_p3	TGGTCGTAGGTTCAAGTCCT	GGCCAAACGACCAAACAATC	50.0	50.0	58.0	58.2	1410 bp	0.333333	51.5
dsbD_p4	AATCGTTTTATGCGGACGGA	CGAGAAAACTTCCAGCGTCT	45.0	50.0	58.0	58.2	1504 bp	0.333333	51.5
dsbD_p5	AATCGTTTTATGCGGACGGA	GCCAAACGACCAAACAATCC	45.0	50.0	58.0	58.2	1669 bp	0.333333	51.5