Hotspot

dnaB

Genome · 143811-145574 · 1764 bp

Back to Caloglossa

Pi 0.274

MarkerSeek score 80.70

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1764
variable_sites: 679
pi: 0.274
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TTTGAACTGGTGACACGAGG	GCTGAAACGGCAACAGAAAA	50.0	45.0	58.1	57.8	2312 bp	0.333333	51.3
dnaB_p2	ACCCTAGTTATGGTGCTCGT	GCTGAAACGGCAACAGAAAA	50.0	45.0	58.1	57.8	2524 bp	0.333333	50.6
dnaB_p3	TTCAGTCCACTGCTCTACCT	GCTGAAACGGCAACAGAAAA	50.0	45.0	57.7	57.8	2289 bp	0.333333	49.2
dnaB_p4	TTTGAACTGGTGACACGAGG	ACAAAATTGCTGAAACGGCA	50.0	40.0	58.1	57.4	2320 bp	0.333333	48.2
dnaB_p5	GAACTGGTGACACGAGGATT	GCTGAAACGGCAACAGAAAA	50.0	45.0	57.5	57.8	2309 bp	0.333333	48.1