Hotspot

carA

Genome · 11166-12347 · 1182 bp

Back to Caloglossa

Pi 0.258

MarkerSeek score 81.20

Primer available yes

Label carA

Feature Metadata

label_name: carA
feature_type: gene
region: Genome
length_bp: 1182
variable_sites: 425
pi: 0.258
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
carA_p1	TTTGGAAGCTATTGCCGGTT	AGGCTGGAGTCATCAAGGAA	45.0	50.0	58.1	58.3	1974 bp	0.333333	50.0
carA_p2	ACGCAATGCCTAGTTTTGGA	AGGCTGGAGTCATCAAGGAA	45.0	50.0	58.1	58.3	1563 bp	0.333333	49.9
carA_p3	ATTTGGAAGCTATTGCCGGT	AGGCTGGAGTCATCAAGGAA	45.0	50.0	57.9	58.3	1975 bp	0.333333	49.5
carA_p4	GCCTAGTTTTGGAGGTCGTT	AGGCTGGAGTCATCAAGGAA	50.0	50.0	57.8	58.3	1556 bp	0.333333	49.2
carA_p5	TTTGGAAGCTATTGCCGGTT	GGCTGGAGTCATCAAGGAAA	45.0	50.0	58.1	57.5	1973 bp	0.333333	48.7