Hotspot

ConsOrf5

Genome · 61996-62964 · 969 bp

Back to Caloglossa

Pi 0.326

MarkerSeek score 82.90

Primer available yes

Label ConsOrf5

Feature Metadata

label_name: ConsOrf5
feature_type: gene
region: Genome
length_bp: 969
variable_sites: 418
pi: 0.326
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ConsOrf5_p1	GCTATTGCATCTGGCTGGAT	GACTCATGTGTGACCCGTTT	50.0	50.0	58.1	58.1	1610 bp	0.333333	51.6
ConsOrf5_p2	CCCGCTACCCGCTTATAAAA	GACTCATGTGTGACCCGTTT	50.0	50.0	57.8	58.1	1646 bp	0.333333	50.4
ConsOrf5_p3	CGAACCAGCGGTGTCTTATT	GACTCATGTGTGACCCGTTT	50.0	50.0	58.3	58.1	1589 bp	0.333333	50.1
ConsOrf5_p4	AAGCTATTGCATCTGGCTGG	GACTCATGTGTGACCCGTTT	50.0	50.0	58.3	58.1	1612 bp	0.333333	49.9
ConsOrf5_p5	GCTATTGCATCTGGCTGGAT	ACTCATGTGTGACCCGTTTT	50.0	45.0	58.1	57.7	1609 bp	0.333333	49.9