Hotspot

ndhG-ndhI

SSC · 119712-120074 · 363 bp

Back to Calochortus

Pi 0.0475

MarkerSeek score 72.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 363
variable_sites: 17
pi: 0.0475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAATGAAAAGGCGGAAT	AGACAACCTTGATTCAAGAACA	45.0	36.4	57.8	55.9	520-536 bp	1.0	48.3
ndhG-ndhI_p2	AATACTACTCCCAAGCCCCC	AGACAACCTTGATTCAAGAACA	55.0	36.4	58.5	55.9	483-499 bp	1.0	46.6
ndhG-ndhI_p3	TCCCAATGAAAAGGCGGAAT	AGACAACCTTGATTCAAGAACAA	45.0	34.8	57.8	56.6	520-536 bp	1.0	46.0
ndhG-ndhI_p4	AATACTACTCCCAAGCCCCC	AGACAACCTTGATTCAAGAACAA	55.0	34.8	58.5	56.6	483-499 bp	1.0	44.3
ndhG-ndhI_p5	TCCCAATGAAAAGGCGGAAT	GACAACCTTGATTCAAGAACA	45.0	38.1	57.8	54.3	519-535 bp	1.0	44.2