Hotspot

petD-rpoA

LSC · 77738-77980 · 243 bp

Back to Calochortus

Pi 0.163

MarkerSeek score 76.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 243
variable_sites: 36
pi: 0.163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGATCCATTTGCGACTCCTT	TGCCGTATACATGCCTGTTC	50.0	50.0	58.0	58.1	1066-1079 bp	1.0	84.6
petD-rpoA_p2	GGATCCATTTGCGACTCCTT	AGGGATCGCGGATATCGTAT	50.0	50.0	58.0	57.9	1128-1141 bp	1.0	84.4
petD-rpoA_p3	GTTCTTTCGGGGGATGGATC	TGCCGTATACATGCCTGTTC	55.0	50.0	58.0	58.1	1298-1311 bp	1.0	83.9
petD-rpoA_p4	GTTCTTTCGGGGGATGGATC	AGGGATCGCGGATATCGTAT	55.0	50.0	58.0	57.9	1360-1373 bp	1.0	83.8
petD-rpoA_p5	GTTCTTTCGGGGGATGGATC	AGATACGTAACCGCTCGAGA	55.0	50.0	58.0	58.1	1477-1490 bp	1.0	83.5