Hotspot

rbcL-accD

LSC · 55859-56742 · 884 bp

Back to Calochortus

Pi 0.0255

MarkerSeek score 75.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 22
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGTGAAGCTTGCAAATGGAG	GAGTTCTTCACTTCCACTGCT	50.0	47.6	58.0	57.9	1112-1113 bp	1.0	74.0
rbcL-accD_p2	CGTGAAGCTTGCAAATGGAG	AGTTCTTCACTTCCACTGCT	50.0	45.0	58.0	56.7	1111-1112 bp	1.0	72.5
rbcL-accD_p3	TCGAGTTCGAACCGGTAGAT	GAGTTCTTCACTTCCACTGCT	50.0	47.6	58.3	57.9	1048-1049 bp	1.0	72.3
rbcL-accD_p4	AAGCTTGCAAATGGAGTCCT	GAGTTCTTCACTTCCACTGCT	45.0	47.6	57.7	57.9	1108-1109 bp	1.0	71.8
rbcL-accD_p5	GGCGATCAAATTCGAGTTCG	GAGTTCTTCACTTCCACTGCT	50.0	47.6	57.6	57.9	1059-1060 bp	1.0	71.5