Hotspot

trnM-CAU-atpE

LSC · 51340-51543 · 204 bp

Back to Calochortus

Pi 0.0711

MarkerSeek score 80.50

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 204
variable_sites: 14
pi: 0.0711
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	GCGAATCTAGCTCTCAGACG	50.0	55.0	58.1	58.0	296-301 bp	1.0	84.7
trnM-CAU-atpE_p2	TCCCGAAGTAATGGGTCCTT	GCGAATCTAGCTCTCAGACG	50.0	55.0	58.0	58.0	368-373 bp	1.0	84.0
trnM-CAU-atpE_p3	TTCCCGAAGTAATGGGTCCT	GCGAATCTAGCTCTCAGACG	50.0	55.0	58.0	58.0	369-374 bp	1.0	84.0
trnM-CAU-atpE_p4	TCCCGAAGTAATGGGTCCTT	TGAGGCGAATCTAGCTCTCA	50.0	50.0	58.0	57.9	372-377 bp	1.0	83.7
trnM-CAU-atpE_p5	TTCCCGAAGTAATGGGTCCT	TGAGGCGAATCTAGCTCTCA	50.0	50.0	58.0	57.9	373-378 bp	1.0	83.7