Hotspot

ndhJ-ndhK

LSC · 48213-48297 · 85 bp

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Pi 0.0588

MarkerSeek score 74.70

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 85
variable_sites: 5
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CCTTGGTAATCGAAGCCCAA	AGTGAATCAAGCGGGGTTTT	50.0	45.0	57.9	58.0	193-201 bp	1.0	81.0
ndhJ-ndhK_p2	TTGGTAATCGAAGCCCAAGG	AGTGAATCAAGCGGGGTTTT	50.0	45.0	57.9	58.0	191-199 bp	1.0	81.0
ndhJ-ndhK_p3	TTCCTTGGTAATCGAAGCCC	AGTGAATCAAGCGGGGTTTT	50.0	45.0	57.6	58.0	195-203 bp	1.0	78.7
ndhJ-ndhK_p4	GGTAATCGAAGCCCAAGGAT	AGTGAATCAAGCGGGGTTTT	50.0	45.0	57.4	58.0	189-197 bp	1.0	76.9
ndhJ-ndhK_p5	TGGTAATCGAAGCCCAAGGA	AGTGAATCAAGCGGGGTTTT	50.0	45.0	58.7	58.0	190-198 bp	1.0	76.3