Hotspot

trnF-GAA-ndhJ

LSC · 47153-47735 · 583 bp

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Pi 0.0312

MarkerSeek score 72.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 17
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TTGATGCCCGAAAGTTGGAT	50.0	45.0	58.1	57.8	667-701 bp	1.0	83.9
trnF-GAA-ndhJ_p2	AAAGGTCGGGATAGCTCAGT	TTGATGCCCGAAAGTTGGAT	50.0	45.0	58.1	57.8	710-744 bp	1.0	83.1
trnF-GAA-ndhJ_p3	AAGGTCGGGATAGCTCAGTT	TTGATGCCCGAAAGTTGGAT	50.0	45.0	58.1	57.8	709-743 bp	1.0	83.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGCTG	50.0	55.0	58.1	58.3	661-695 bp	1.0	82.9
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGCTGG	50.0	55.0	58.1	58.3	660-694 bp	1.0	82.9