Hotspot

trnT-UGU-trnL-UAA

LSC · 45642-46327 · 686 bp

Back to Calochortus

Pi 0.0333

MarkerSeek score 73.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 22
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	733-747 bp	1.0	82.0
trnT-UGU-trnL-UAA_p2	AAAGCCAGCTATCGGAATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	778-792 bp	1.0	81.8
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	765-779 bp	1.0	81.0
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	727-741 bp	1.0	80.8
trnT-UGU-trnL-UAA_p5	AAAGCCAGCTATCGGAATCG	CGTCTACCGATTTCGCCATA	50.0	50.0	58.1	57.6	772-786 bp	1.0	80.5