Hotspot

atpF-atpH

LSC · 12937-13535 · 599 bp

Back to Calochortus

Pi 0.0383

MarkerSeek score 75.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 20
pi: 0.0383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AATAGCCCAAGGAAACGCAA	AATTTACGGACTGGTCGTGG	45.0	50.0	58.1	57.9	617-694 bp	1.0	85.1
atpF-atpH_p2	ATAGCCCAAGGAAACGCAAA	AATTTACGGACTGGTCGTGG	45.0	50.0	58.1	57.9	616-693 bp	1.0	85.1
atpF-atpH_p3	GCCCAAGGAAACGCAAAAAT	AATTTACGGACTGGTCGTGG	45.0	50.0	58.1	57.9	613-690 bp	1.0	84.8
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	AATTTACGGACTGGTCGTGG	50.0	50.0	57.9	57.9	646-723 bp	1.0	84.7
atpF-atpH_p5	ATAGCCCAAGGAAACGCAAA	TCGTGGCATTAGCGCTTTTA	45.0	45.0	58.1	58.3	602-679 bp	1.0	83.7