Hotspot

chlL-trnH-GUG

Genome · 126444-126589 · 146 bp

Back to Calocedrus

Pi 0.0286

MarkerSeek score 72.80

Primer available yes

Label chlL-trnH-GUG

Feature Metadata

label_name: chlL-trnH-GUG
feature_type: igs
region: Genome
length_bp: 146
variable_sites: 4
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL-trnH-GUG_p1	CCGATTCCGCCTTTTCCATA	CCAAGGCAGTGGATTGTGAA	50.0	50.0	58.0	58.4	231-236 bp	1.0	80.1
chlL-trnH-GUG_p2	CCGATTCCGCCTTTTCCATA	GTAGCCAAGTGGTTCCAAGG	50.0	55.0	58.0	58.5	245-250 bp	1.0	79.2
chlL-trnH-GUG_p3	TTACCGATTCCGCCTTTTCC	CCAAGGCAGTGGATTGTGAA	50.0	50.0	58.3	58.4	234-239 bp	1.0	78.3
chlL-trnH-GUG_p4	CCGCCTTTTCCATAAACTGC	CCAAGGCAGTGGATTGTGAA	50.0	50.0	57.7	58.4	225-230 bp	1.0	78.2
chlL-trnH-GUG_p5	TTACCGATTCCGCCTTTTCC	GTAGCCAAGTGGTTCCAAGG	50.0	55.0	58.3	58.5	248-253 bp	1.0	77.4