Hotspot

rps12-clpP

Genome · 101347-102486 · 1140 bp

Back to Calocedrus

Pi 0.0338

MarkerSeek score 81.50

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 1140
variable_sites: 35
pi: 0.0338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GCATACTCCCTTCCGTTGAG	GCAGGTTGTTTTTCTAGCGC	55.0	50.0	58.1	58.3	1254-1319 bp	1.0	83.2
rps12-clpP_p2	ATTGGCTGTCTCGCATTTCT	GCAGGTTGTTTTTCTAGCGC	45.0	50.0	57.9	58.3	1196-1261 bp	1.0	82.8
rps12-clpP_p3	CATACTCCCTTCCGTTGAGG	GCAGGTTGTTTTTCTAGCGC	55.0	50.0	57.4	58.3	1253-1318 bp	1.0	78.8
rps12-clpP_p4	TACACCCTAGCGCATACTCC	GCAGGTTGTTTTTCTAGCGC	55.0	50.0	58.7	58.3	1265-1330 bp	1.0	78.3
rps12-clpP_p5	TGGCTGTCTCGCATTTCTAA	GCAGGTTGTTTTTCTAGCGC	45.0	50.0	57.2	58.3	1194-1259 bp	1.0	77.7