Hotspot

petA-psbJ

Genome · 96025-96490 · 466 bp

Back to Calocedrus

Pi 0.0265

MarkerSeek score 65.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 466
variable_sites: 11
pi: 0.0265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTCGTTTTAGCACAG	ACCGGATTAGGATCTTCCTTG	47.6	47.6	57.9	56.9	528-574 bp	1.0	59.7
petA-psbJ_p2	GCATCTGTCGTTTTAGCACAG	CCGGATTAGGATCTTCCTTGT	47.6	47.6	57.9	56.9	527-573 bp	1.0	59.7
petA-psbJ_p3	AGCATCTGTCGTTTTAGCACA	ACCGGATTAGGATCTTCCTTG	42.9	47.6	58.2	56.9	529-575 bp	1.0	58.7
petA-psbJ_p4	AGCATCTGTCGTTTTAGCACA	CCGGATTAGGATCTTCCTTGT	42.9	47.6	58.2	56.9	528-574 bp	1.0	58.7
petA-psbJ_p5	GCATCTGTCGTTTTAGCACA	CCGGATTAGGATCTTCCTTGT	45.0	47.6	56.7	56.9	527-573 bp	1.0	58.6