Hotspot

trnI-GAU-rrn16

Genome · 62707-63213 · 507 bp

Back to Calocedrus

Pi 0.0344

MarkerSeek score 70.80

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: Genome
length_bp: 507
variable_sites: 17
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	GCGTTGATGAGCACATTGAA	CAAGGTAGCCGTACTGGAAG	45.0	55.0	57.4	57.7	649-653 bp	1.0	76.4
trnI-GAU-rrn16_p2	GCGTTGATGAGCACATTGAA	AGTGACTGGAGTGAAGTCGT	45.0	50.0	57.4	58.3	671-675 bp	1.0	76.2
trnI-GAU-rrn16_p3	GCGTTGATGAGCACATTGAA	AAGTCGTAACAAGGTAGCCG	45.0	50.0	57.4	57.6	658-662 bp	1.0	75.8
trnI-GAU-rrn16_p4	GCGTTGATGAGCACATTGAA	AACAAGGTAGCCGTACTGGA	45.0	50.0	57.4	58.4	651-655 bp	1.0	75.8
trnI-GAU-rrn16_p5	GCGTTGATGAGCACATTGAA	ACAAGGTAGCCGTACTGGAA	45.0	50.0	57.4	58.4	650-654 bp	1.0	75.8