Hotspot

trnD-GUC-trnY-GUA

Genome · 28493-28705 · 213 bp

Back to Calocedrus

Pi 0.0235
MarkerSeek score 66.00
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
Genome
length_bp
213
variable_sites
5
pi
0.0235
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 TTGACAGGGCGGTACTCTAA CCTACGCTGGTTCAAATCCA 50.0 50.0 58.1 57.9 282 bp 1.0 80.0
trnD-GUC-trnY-GUA_p2 TTGACAGGGCGGTACTCTAA ATGCCTACGCTGGTTCAAAT 50.0 45.0 58.1 57.9 285 bp 1.0 79.8
trnD-GUC-trnY-GUA_p3 AGGGCGGTACTCTAACCAAT CCTACGCTGGTTCAAATCCA 50.0 50.0 58.1 57.9 277 bp 1.0 79.6
trnD-GUC-trnY-GUA_p4 AGGGCGGTACTCTAACCAAT ATGCCTACGCTGGTTCAAAT 50.0 45.0 58.1 57.9 280 bp 1.0 79.4
trnD-GUC-trnY-GUA_p5 TTGACAGGGCGGTACTCTAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.1 58.4 313 bp 1.0 77.9