Hotspot

ycf2

Genome · 39473-46882 · 7410 bp

Back to Callitropsis

Pi 0.00585

MarkerSeek score 48.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 7410
variable_sites: 43
pi: 0.00585
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CAGGTCGAAAAATAGGGCGA	AACGTATTGGCACCTGTTGT	50.0	45.0	58.0	58.0	916 bp	0.5	59.7
ycf2_p2	CCAAACCAACCCCAATGTTG	AACGTATTGGCACCTGTTGT	50.0	45.0	57.7	58.0	1085 bp	0.5	57.8
ycf2_p3	ACAGGTCGAAAAATAGGGCG	AACGTATTGGCACCTGTTGT	50.0	45.0	58.3	58.0	917 bp	0.5	57.7
ycf2_p4	CATTAAGCTTGGCCCAGAGA	AACGTATTGGCACCTGTTGT	50.0	45.0	57.6	58.0	1174 bp	0.5	56.4
ycf2_p5	TCATTAAGCTTGGCCCAGAG	AACGTATTGGCACCTGTTGT	50.0	45.0	57.6	58.0	1175 bp	0.5	56.4