Hotspot

ycf1

Genome · 31482-38507 · 7026 bp

Back to Callitropsis

Pi 0.00769

MarkerSeek score 42.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 7026
variable_sites: 54
pi: 0.00769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCATTCGGGAACTTGAAACAG	TGGATACCGAGACCAGAGTC	47.6	55.0	57.4	57.9	327 bp	1.0	68.8
ycf1_p2	CCATTCGGGAACTTGAAACAG	ACCATTGGATACCGAGACCA	47.6	50.0	57.4	58.1	332 bp	1.0	68.2
ycf1_p3	CCATTCGGGAACTTGAAACA	TGGATACCGAGACCAGAGTC	45.0	55.0	56.2	57.9	327 bp	1.0	67.3
ycf1_p4	CCATTCGGGAACTTGAAACA	ACCATTGGATACCGAGACCA	45.0	50.0	56.2	58.1	332 bp	1.0	66.7
ycf1_p5	CCATTCGGGAACTTGAAACAG	TTGGATACCGAGACCAGAGT	47.6	50.0	57.4	57.5	328 bp	1.0	65.0