Hotspot

ndhC-trnV-UAC

Genome · 122949-123709 · 761 bp

Back to Callitropsis

Pi 0.00404
MarkerSeek score 63.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
Genome
length_bp
761
variable_sites
3
pi
0.00404
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGAGAATGCCAGAATCGGAA TGCATGTTGGGTTCTTGGAA 45.0 45.0 56.9 57.9 935-937 bp 1.0 72.6
ndhC-trnV-UAC_p2 TTGAGAATGCCAGAATCGGA TGCATGTTGGGTTCTTGGAA 45.0 45.0 56.9 57.9 936-938 bp 1.0 72.6
ndhC-trnV-UAC_p3 TGAGAATGCCAGAATCGGAA ATGCATGTTGGGTTCTTGGA 45.0 45.0 56.9 57.7 936-938 bp 1.0 70.8
ndhC-trnV-UAC_p4 TTGAGAATGCCAGAATCGGA ATGCATGTTGGGTTCTTGGA 45.0 45.0 56.9 57.7 937-939 bp 1.0 70.8
ndhC-trnV-UAC_p5 TTGAGAATGCCAGAATCGGAA TGCATGTTGGGTTCTTGGAA 42.9 45.0 57.7 57.9 936-938 bp 1.0 70.5