Hotspot

trnH-GUG-chlL

Genome · 117848-118496 · 649 bp

Back to Callitropsis

Pi 0.00929

MarkerSeek score 64.50

Primer available yes

Label trnH-GUG-chlL

Feature Metadata

label_name: trnH-GUG-chlL
feature_type: igs
region: Genome
length_bp: 649
variable_sites: 6
pi: 0.00929
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-chlL_p1	CCAAGGCAGTGGATTGTGAA	CCGATTCCGCCTTTTCCATA	50.0	50.0	58.4	58.0	738-740 bp	1.0	77.6
trnH-GUG-chlL_p2	GTAGCCAAGTGGTTCCAAGG	CCGATTCCGCCTTTTCCATA	55.0	50.0	58.5	58.0	752-754 bp	1.0	76.9
trnH-GUG-chlL_p3	CCAAGGCAGTGGATTGTGAA	TTACCGATTCCGCCTTTTCC	50.0	50.0	58.4	58.3	741-743 bp	1.0	75.9
trnH-GUG-chlL_p4	CCAAGGCAGTGGATTGTGAA	CCGCCTTTTCCATAAACTGC	50.0	50.0	58.4	57.7	732-734 bp	1.0	75.7
trnH-GUG-chlL_p5	GTAGCCAAGTGGTTCCAAGG	TTACCGATTCCGCCTTTTCC	55.0	50.0	58.5	58.3	755-757 bp	1.0	75.2