Hotspot

ndhF-rpl32

Genome · 64180-64938 · 759 bp

Back to Callitropsis

Pi 0.0225
MarkerSeek score 63.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
Genome
length_bp
759
variable_sites
17
pi
0.0225
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CACGACCCGAGCATATTTGT TGCGTCCGAATTTGCTTTTT 50.0 40.0 58.3 57.5 876-924 bp 1.0 75.0
ndhF-rpl32_p2 CACGACCCGAGCATATTTGT TTGCGTCCGAATTTGCTTTT 50.0 40.0 58.3 57.5 877-925 bp 1.0 75.0
ndhF-rpl32_p3 CCGATTGGTACAGAAGCTGA TGCGTCCGAATTTGCTTTTT 50.0 40.0 57.3 57.5 911-959 bp 1.0 72.3
ndhF-rpl32_p4 CCGATTGGTACAGAAGCTGA TTGCGTCCGAATTTGCTTTT 50.0 40.0 57.3 57.5 912-960 bp 1.0 72.3
ndhF-rpl32_p5 TCCGATTGGTACAGAAGCTG TGCGTCCGAATTTGCTTTTT 50.0 40.0 57.3 57.5 912-960 bp 1.0 72.3