Hotspot

rrn16-trnI-GAU

Genome · 54188-55136 · 949 bp

Back to Callitropsis

Pi 0.0286

MarkerSeek score 75.60

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: Genome
length_bp: 949
variable_sites: 27
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	AGTGACTGGAGTGAAGTCGT	GCGTTGATGAGCACATTGAA	50.0	45.0	58.3	57.4	1088-1175 bp	1.0	74.8
rrn16-trnI-GAU_p2	AAGTCGTAACAAGGTAGCCG	GCGTTGATGAGCACATTGAA	50.0	45.0	57.6	57.4	1075-1162 bp	1.0	74.4
rrn16-trnI-GAU_p3	GGCTAGTGACTGGAGTGAAG	GCGTTGATGAGCACATTGAA	55.0	45.0	57.3	57.4	1092-1179 bp	1.0	71.9
rrn16-trnI-GAU_p4	TGCCATGCTCGAAGTCATTA	GCGTTGATGAGCACATTGAA	45.0	45.0	57.3	57.4	1145-1232 bp	1.0	71.6
rrn16-trnI-GAU_p5	AGTCGTAACAAGGTAGCCGT	GCGTTGATGAGCACATTGAA	50.0	45.0	58.7	57.4	1074-1161 bp	1.0	71.3