Hotspot

trnP-GGG-trnL-UAG

Genome · 30092-30227 · 136 bp

Back to Callitropsis

Pi 0.0403

MarkerSeek score 64.70

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: Genome
length_bp: 136
variable_sites: 5
pi: 0.0403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	GAGCTTTCCTTCTACACGGG	CGAATAGAGCGAACGGGAAA	55.0	50.0	58.0	58.1	1270-1282 bp	1.0	81.1
trnP-GGG-trnL-UAG_p2	GGAGCTTTCCTTCTACACGG	CGAATAGAGCGAACGGGAAA	55.0	50.0	58.0	58.1	1271-1283 bp	1.0	81.1
trnP-GGG-trnL-UAG_p3	GGGAGCTTTCCTTCTACACG	CGAATAGAGCGAACGGGAAA	55.0	50.0	58.0	58.1	1272-1284 bp	1.0	81.1
trnP-GGG-trnL-UAG_p4	GAGCTTTCCTTCTACACGGG	GTGATTGCACCTAACCACCA	55.0	50.0	58.0	58.1	1389-1401 bp	1.0	80.9
trnP-GGG-trnL-UAG_p5	GGAGCTTTCCTTCTACACGG	GTGATTGCACCTAACCACCA	55.0	50.0	58.0	58.1	1390-1402 bp	1.0	80.9