Hotspot

psbD

Genome · 67301-68362 · 1062 bp

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Pi 0.523

MarkerSeek score 80.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 463
pi: 0.523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	AGTGTCCACAATTTCCCCAC	AGCAGGGAGAAAGAAAAGCT	50.0	45.0	58.0	57.0	1613 bp	0.5	52.2
psbD_p2	CCCACGCAAAAGGTTTTGTT	AGCAGGGAGAAAGAAAAGCT	45.0	45.0	58.0	57.0	2067 bp	0.5	52.1
psbD_p3	GGGTCCAATTAGTGCGTGAT	AGCAGGGAGAAAGAAAAGCT	50.0	45.0	58.0	57.0	1700 bp	0.5	52.0
psbD_p4	AGTGGTTTCTTGGTCACGAC	AGCAGGGAGAAAGAAAAGCT	50.0	45.0	58.1	57.0	1382 bp	0.5	51.9
psbD_p5	CAGTGGTTTCTTGGTCACGA	AGCAGGGAGAAAGAAAAGCT	50.0	45.0	58.1	57.0	1383 bp	0.5	51.8