Hotspot

psaA

Genome · 59852-62101 · 2250 bp

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Pi 0.491

MarkerSeek score 77.70

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: Genome
length_bp: 2250
variable_sites: 878
pi: 0.491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	TCGCTCGAGTTCTGAATTGG	CCGGTTGACCAAAATGAGGA	50.0	50.0	58.0	58.1	2712 bp	0.5	59.2
psaA_p2	TCGCTCGAGTTCTGAATTGG	ACTAAATGTCCCGTCCAAGC	50.0	50.0	58.0	57.9	2998 bp	0.5	59.1
psaA_p3	TCGCTCGAGTTCTGAATTGG	ACCACCACTGATAAACACCG	50.0	50.0	58.0	57.8	2787 bp	0.5	58.5
psaA_p4	TCGCTCGAGTTCTGAATTGG	CTCGGGTAAAGGCTTCTACG	50.0	55.0	58.0	57.8	2733 bp	0.5	58.3
psaA_p5	CGGTCCATTGAGCACCTATC	CCGGTTGACCAAAATGAGGA	55.0	50.0	58.1	58.1	2786 bp	0.5	58.2