Hotspot

ndhJ

Genome · 71226-71750 · 525 bp

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Pi 0.516

MarkerSeek score 81.20

Primer available yes

Label ndhJ

Feature Metadata

label_name: ndhJ
feature_type: gene
region: Genome
length_bp: 525
variable_sites: 249
pi: 0.516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ_p1	CCAAATACTGGCCAGGCTAG	GTTCGCGGAGTGGATAAGTT	55.0	50.0	58.0	58.0	1584 bp	0.5	59.7
ndhJ_p2	CCAAATACTGGCCAGGCTAG	TTTGGTGCCAAGATCCAGTC	55.0	50.0	58.0	58.1	1765 bp	0.5	59.1
ndhJ_p3	CCAAATACTGGCCAGGCTAG	CTACTTGCCAGGTTGTCCTC	55.0	55.0	58.0	57.9	1549 bp	0.5	59.0
ndhJ_p4	CCAAATACTGGCCAGGCTAG	CCGGAACTGTGACCATGAAA	55.0	50.0	58.0	58.1	1718 bp	0.5	58.9
ndhJ_p5	CCAAATACTGGCCAGGCTAG	TAGGCAAGCCGACCTACTTA	55.0	50.0	58.0	57.9	1744 bp	0.5	58.6