Hotspot

chlN

Genome · 74069-75427 · 1359 bp

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Pi 0.432

MarkerSeek score 80.90

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 1359
variable_sites: 496
pi: 0.432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	TCCCCAACTACCAGCACTAA	GCTCCGTTGAACTATGCAGA	50.0	50.0	58.0	58.0	2319 bp	0.5	59.7
chlN_p2	TACATCCCCAACTACCAGCA	GCTCCGTTGAACTATGCAGA	50.0	50.0	58.0	58.0	2323 bp	0.5	59.6
chlN_p3	TCCCCAACTACCAGCACTAA	GTGGTTTGTGGAGGTTTTGC	50.0	50.0	58.0	58.1	2340 bp	0.5	59.2
chlN_p4	TACATCCCCAACTACCAGCA	GTGGTTTGTGGAGGTTTTGC	50.0	50.0	58.0	58.1	2344 bp	0.5	59.2
chlN_p5	TCCCCAACTACCAGCACTAA	TGTAGAAGCCTGTCCAATGC	50.0	50.0	58.0	57.9	2146 bp	0.5	58.8