Hotspot

chlL

Genome · 75449-76321 · 873 bp

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Pi 0.457

MarkerSeek score 80.60

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: Genome
length_bp: 873
variable_sites: 336
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	TGGACGGCAAAGATCCAAAA	TTGCTTGGCTTCCTCCATTT	45.0	45.0	58.0	58.0	1664 bp	0.5	59.8
chlL_p2	TGGACGGCAAAGATCCAAAA	TTTGCTTGGCTTCCTCCATT	45.0	45.0	58.0	58.0	1665 bp	0.5	59.8
chlL_p3	TTGGACGGCAAAGATCCAAA	TTGCTTGGCTTCCTCCATTT	45.0	45.0	58.0	58.0	1665 bp	0.5	59.8
chlL_p4	TTGGACGGCAAAGATCCAAA	TTTGCTTGGCTTCCTCCATT	45.0	45.0	58.0	58.0	1666 bp	0.5	59.8
chlL_p5	TTCTAGTTTGGGCGCCATAC	TTGCTTGGCTTCCTCCATTT	50.0	45.0	58.0	58.0	1435 bp	0.5	59.6