Hotspot

accD

Genome · 102875-104908 · 2034 bp

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Pi 0.156

MarkerSeek score 82.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 2034
variable_sites: 305
pi: 0.156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAAGGACGTGTTATCTCGGC	AGTGTGGACTATTCGCGAAC	50.0	50.0	58.0	58.0	2670 bp	0.5	59.9
accD_p2	AAAGGACGTGTTATCTCGGC	TATTCGCGAACGTGGTTCTT	50.0	45.0	58.0	57.9	2661 bp	0.5	59.4
accD_p3	CAAGAGCGTAGTCCTGCTTT	AGTGTGGACTATTCGCGAAC	50.0	50.0	57.9	58.0	2453 bp	0.5	59.2
accD_p4	AAAGGACGTGTTATCTCGGC	GCTAACGGAGAAAGAGGCAA	50.0	50.0	58.0	57.9	2891 bp	0.5	59.2
accD_p5	GTGTTTTGGTTTGGTTGGCA	AGTGTGGACTATTCGCGAAC	45.0	50.0	57.9	58.0	2842 bp	0.5	59.1