Hotspot

psbJ-trnK-UUU

Genome · 109801-110253 · 453 bp

Back to Callitris

Pi 0.0569

MarkerSeek score 80.20

Primer available yes

Label psbJ-trnK-UUU

Feature Metadata

label_name: psbJ-trnK-UUU
feature_type: igs
region: Genome
length_bp: 453
variable_sites: 24
pi: 0.0569
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-trnK-UUU_p1	CCGGAAGAATCCCTCTTTGG	AGAGCGGATAATCCACTCCA	55.0	50.0	58.0	57.9	610-619 bp	1.0	86.1
psbJ-trnK-UUU_p2	CCGGAAGAATCCCTCTTTGG	ATCCACTCCATCCGATGAGT	55.0	50.0	58.0	57.9	600-609 bp	1.0	86.1
psbJ-trnK-UUU_p3	CCGGAAGAATCCCTCTTTGG	GCGGATAATCCACTCCATCC	55.0	55.0	58.0	57.9	607-616 bp	1.0	86.0
psbJ-trnK-UUU_p4	CCGGAAGAATCCCTCTTTGG	TCCACTCCATCCGATGAGTT	55.0	50.0	58.0	58.1	599-608 bp	1.0	85.8
psbJ-trnK-UUU_p5	CCGGAAGAATCCCTCTTTGG	CACTCCATCCGATGAGTTCC	55.0	55.0	58.0	57.8	597-606 bp	1.0	85.0